2.6 Biosynthesis of proteoglycansrequires several modifying enzymes inaddition to glycosyltransferases
2.6 Biosynthesis of proteoglycans requires several modifying enzymes in addition to glycosyltransferases
o Target sequences for GAG attachment: serine-glycineo Not all of them are derivatized in this wayo Additional signals in protein structure definesattachment sitesO Addition of chains in clusters results fromprocessive activity of the core xylosyltransferaseo Adjacent Ser-Gly are targets for the enzyme afterglycosylation of an initial sequenceo A cluster of negative charges directs the enzyme
o Target-sequences-for-GAG-a>achment:-serine9 glycine- o Not-all-of-them-are-deriva7zed-in-this-wayo Addi7onal-signals-in-protein-structure-definesa>achment-siteso Addi7on-of-chains-in-clusters-results-fromprocessive-ac7vity-of-the-core-xylosyltransferase o Adjacent-Ser9Gly-are-targets-for-the-enzyme-a`erglycosyla7on-of-an-ini7al-sequenceo A-cluster-of-nega7ve-charges-directs-the-enzyme-